In this Research, the effects of different nitrogen fertilizer rates in wheat parents (Azar-2 cultivar) on seed vigour, seedlings establishment and grain yield were evaluated. Laboratory tests based on CRD with 5 treatments and 4 replications along with a field experiment based on completely randomized block design with five treatments and four replications were carried out in Dryland Agricultural Research Institute (DARI), Maragheh in 2011-2012. In this research the seeds of wheat that they were produced with various rates of nitrogen fertilizer (0, 30, 60, 90 and 120 kg/ha) were used. The nitrogen contents of seeds on the basis of percentage in the parent plants in the different rates of nitrogen fertilizer were measured. In addition, the measurement of nitrogen indicated that the seeds which were produced in the different rates of nitrogen had a significant difference among themselves. Laboratory results showed that electrical conductivity (EC) of seeds leakages, dry weight of root and shoot of seedling, seedling dry weight, germination percentage and the mean of germination time were significantly affected by nitrogen. Means comparison data showed that the highest electrical conductivity (EC) of seeds leakages, germination rate, radicle, plumule and seedling dry weight, seed nitrogen percentage, were related to 90 and 120 kg.ha of nitrogen use in parent plants. The field results showed that protein percentage, emergence rate, mean time of emergence, percentage of seedlings establishment, green cover percentage of flowering, number of spik per m² and number of fertile tillers and biologic yield were significantly affected by nitrogen use on the parent plants. These results indicated that the use of 90 kg.ha nitrogen fertilizer in the parent plants for Azar-2 had positive and significant effect on seed vigour, seedlings establishment and grain yield.