In order to investigate the effects of planting pattern and split application of nitrogen fertilizer on qualitative traits and grain yield of rice (var. Koohsar) in second cropping, a field experiment was conducted as split-plot in randomized complete block design with three replications in Research Farm of Amol University in 2013. The treatments included planting pattern in three levels: D1:25×25, D2: 20×20 and D3: 30×10 cm² as main plot and time of nitrogen split application in five levels (T1: 100% basal fertilizer, T2: 50% basal + 50% tillering stage, T3: 50% basal + 50% panicle initiation stage, T4: 33.33% basal + 33.33% tillering stage + 33.33% panicle initiation stage and T5: 33.33% basal + 33.33% tillering stage + 33.33% full heading stage) as sub plot were studied. Results showed that decreasing of planting density (25×25 cm) and increasing of N split, augmented the qualitative traits. But, with the increasing planting density (30×10 cm), the grain yield increased by 13.8%. Considering interaction effects, the longest grain before cooking was observed in treatment D1T4. The maximum grain yield (3874 kg.ha^-1) was observed in treatment T5 split. It could be concluded that the planting pattern of 25×25 cm and splitting of nitrogen fertilizer application in vegetative and reproductive stages, may improve the qualitative yield. However, the planting pattern of 30×10 cm and T3, T4 and T5 split cause the increase of quantitative yield of rice (var. Koohsar) in the second cropping.